Research Papers On Image Processing 2013 Nfl

1Departamento de Ciencias Computacionales, Universidad de Guadalajara, CUCEI, Avenida Revolución 1500, Guadalajara, Jal, Mexico
2Departamento de Ingeniería de Software e Inteligencia Artificial, Facultad Informática, Universidad Complutense, 28040 Madrid, Spain
3Institut für Informatik, Freie Univerisität Berlin, Arnimallee 7, 14195 Berlin, Germany

Copyright © 2013 Erik Cuevas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Vision in general and images in particular have always played an essential role in human life. In the past they were, today they are, and in the future they will continue to be one of our most important information carriers. Recent advances in digital imaging and computer hardware technology have led to an explosion in the use of digital images in a variety of scientific and engineering applications. These applications result from the interaction between fundamental scientific research on the one hand and the development of new and high-standard technology on the other.

Computational intelligence has emerged as a powerful tool for information processing, decision making, and knowledge management. The techniques of computational intelligence have been successfully developed in areas such as neural networks, fuzzy systems, and evolutionary algorithms. It is predictable that in the near future computational intelligence will play a more important role in tackling several engineering problems.

Image processing is a very important research area. Classical image processing methods often face great difficulties while dealing with images containing noise and distortions. Under such conditions, the use of computational intelligence approaches has been recently extended to address challenging real-world image processing problems. The interest on the subject among researchers and developers is increasing day by day as it is branded by huge volumes of research works that get published in leading international journals and international conference proceedings.

When the idea of this special issue was first conceived, the goal was to mainly expose the readers to the cutting-edge research and applications that are going on across the domain of image processing where contemporary computational intelligence techniques can be or have been successfully employed.

The special issue received 49 high-quality submissions from different countries all over the world. All submitted papers followed the same standard of peer-review by at least three independent reviewers, as it is applied to regular submissions to Mathematical Problems in Engineering. Due to the limited space, 16 papers were finally included. The primary guideline was to demonstrate the wide scope of computational intelligence algorithms and their applications to image processing problems.

The paper authored by K. haransiriphaisan et al. presents a study of the performance of a family of artificial bee colony algorithms, namely, the standard ABC, ABC/best/1, ABC/best/2, IABC/best/1, IABC/rand/1, and CABC and some particle swarm optimization-based algorithms for searching multilevel thresholding in image segmentation. The strategy for an onlooker bee to select an employee bee was modified for a new alternative mechanism. The experimental results showed that IABC/best/1 outperformed the other techniques, when all of those algorithms were applied for multilevel image segmentation.

The paper by B. Ojeda-Magaña et al. proposed the concept of typicality from the field of cognitive psychology to categorize data into concepts. The idea is to apply such concepts into the interpretation of numerical data in color images for segmentation purposes.

S. E. Gonzalez-Reyna et al. propose a road sign detection method based on oriented gradient maps and the Karhunen-Loeve transform. The proposed algorithm is able to reach good classification accuracy by using a reduced amount of attributes compared to other similar state-of-the-artmethods.

I. Cruz-Aceves et al. present an automatic image segmentation method based on the theory of active contour models and estimation of distribution algorithms. The proposed method uses the univariate marginal distribution model to infer statistical dependencies between the control points on different active contours. These contours have been generated through an alignment process of reference shape priors aiming to increase the exploration and exploitation capabilities regarding different interactive segmentation techniques. The algorithm is applied in the segmentation of images with different complexity levels.

The paper by S.-F. Tu and C.-S. Hsu proposed the use of a modified version of the popular differential evolution (DE) to construct an optimal least Significant Bit (LSB) matrix for information hiding in images.

The paper authored by L. Shen et al. presents a robust Bayesian approach for multisensor image matching. The method is implemented by using the gradient prior information which is estimate by the kernel density estimated (KDE) method, whereas the likelihood is modeled according to the distance of orientations. Experiments on several groups of multisensor images show that the proposed method outperforms the standard methods in terms of robustness and accuracy.

V. Magudeeswaran and C. G. Ravichandran introduce a new method for histogram equalization based on fuzzy logic. The method consists of two stages. First, the fuzzy histogram is computed based on fuzzy set theory to handle the inexactness of gray level values. In the second stage, the fuzzy histogram is divided into two subhistograms based on the median value of the original image and then equalizes them independently to preserve image brightness. The performance of the proposed approach was evaluated considering qualitative and quantitative performance indexes.

S. Chen et al. present a universal noise removal algorithm by combining spatial gradient and a new impulse statistic into the trilateral filter. Simulation results show that the proposed method has a high denoising rate, in particular for salt-and-pepper noise. It is also demonstrated that the computational complexity of the proposed method is less than many other mixed noise filters.

The paper by Y. R. Tabar and I. Ulusoy presented a study where several segmentation methods are implemented and applied to segment the facial masseter tissue from magnetic resonance images. In the study, a new approach is also proposed where unlabeled prior information from a set of MR images is used to segment masseter tissue within a probabilistic framework. The proposed method uses only a seed point that indicates the target tissue and performs automatic segmentation for the selected tissue avoiding the use of a labeled training set. The segmentation results of all methods are validated and compared to particularly discuss the influences of labeled or unlabeled prior information and initialization.

I.-D. Lee et al. present a reversible data hiding scheme to embed secret data into a side matched vector quantization (SMVQ)-compressed image. By using this strategy, fewer bits can be utilized to encode SMVQ indexes with very small values. Experimental results show that the performance of the proposed scheme is superior to those from former data hiding schemes for VQ-based, VQ/SMVQ-based, and search order coding (SOC)-based compressed images.

P. Han et al. present a computational intelligence method by using wavelet optical flow and hybrid linear-nonlinear classifier for object detection. The algorithm can accurately detect moving objects with variable speeds in a scene. Experimental results confirm that the proposed object detection method possesses an improved accuracy and computation efficiency over other state-of-the-art methods.

The paper by Z. Fan et al. proposes a filter algorithm that combines multi-objective genetic algorithm (MOGA) and shearlet transformation to remove noise in images. The approach considers five stages. First, the multiscale wavelet decomposition is applied to the target image. Second, the MOGA target function is constructed by evaluation methods, such as signal to noise ratio (SNR) and mean square errors (MSE). Then, MOGA is used to calculate optimal coefficients of shearlet wavelet threshold value in different scales and different orientations. Finally, the noise-free image is obtained through inverse wavelet transform. Experimental simulations show that the proposed algorithm eliminates noise more effectively and yields better peak signal noise ratio (PSNR) gains compared to other traditional filters.

C. A. Atoche et al. present a parallel tool for large-scale image enhancement/reconstruction and postprocessing for Radar/SAR sensor systems. The proposed parallel tool performs the following intelligent processing steps: image formation for the application of different system-level effects of image degradation with a particular remote sensing (RS) system and simulation of random noising effects, enhancement/reconstruction by employing nonparametric robust high-resolution techniques, and image post processing using the fuzzy anisotropic diffusion technique which incorporates a better edge-preserving noise removal effect and faster diffusion process. To verify the performance implementation of the proposed parallel framework, the processing steps were developed and specifically tested on graphic processor units (GPUs).

The paper authored by E. Cuevas et al. presents an algorithm for the automatic detection of circular shapes from complicated and noisy images with no consideration of the conventional Hough transform principles. The proposed algorithm is based on a newly developed evolutionary algorithm called the adaptive population with reduced evaluations (APRE). The approach reduces the number of function evaluations through the use of two mechanisms: (1) adapting dynamically the size of the population and (2) incorporating a fitness calculation strategy which decides whether the calculation or estimation of the new generated individuals is feasible. As a result, the method can substantially reduce the number of function evaluations, yet preserving the good search capabilities of an evolutionary approach. Experimental results over several synthetic and natural images, with a varying range of complexity, validate the efficiency of the proposed technique with regard to accuracy, speed, and robustness.

B.-Y Park et al. propose a multilabel segmentation that aims to divide a texture image into multiple regions based on a homogeneity condition using local entropy measured at varying scales. For multi-label segmentation, a bipartitioning segmentation scheme is recursively applied to confined regions obtained by previous segmentation steps. On the other hand, the entropy is employed to determine the spatial regularity of elementary texture structures. Experimental results on a variety of texture images demonstrate the efficiency and robustness of the proposed algorithm.

The paper by Haisheng Song et al. presented a neural network (NN) system to classify remote sensing images. The approach uses genetic algorithm to generate the initial structure of NN. Then, weights adjustments are provided by the traditional backpropagation algorithm. Finally, a hybrid algorithm is used to execute classification on remote sensing images. Results show that the hybrid algorithm outperforms other similar approaches.


Finally, we would like to express our gratitude to all of the authors for their contributions and the reviewers for their effort providing valuable comments and feedback. We hope this special issue offers a comprehensive and timely view of the area of applications of computational intelligence in image processing and that it will grant stimulation for further research.

Erik Cuevas
Daniel Zaldívar
Gonzalo Pajares
Marco Perez-Cisneros
Raul Rojas


All animal experiments were conducted according to the Guide of the Committee of Use of Laboratory Animals for Teaching and Research (CULATR) of The University of Hong Kong, Animal Ethics Committee of Shenzhen Institutes of Advanced Technology at Chinese Academy of Sciences, and Nanjing Normal University Animal Research Ethic Committee. The study has been approved by Animal Research Ethic Committee in The University of Hong Kong, Shenzhen Institutes of Advanced Technology at Chinese Academy of Sciences and Nanjing Normal University.


40 adult male Sprague–Dawley (SD) rats (220–250 g, aged 8–10 weeks) were used in the experiments. The animals were housed with food and water ad libitum under 12-hour light/12-hour dark cycle (7:00 AM–7:00 PM). For the surgery, the animals were anesthetized and maintained with muscular injections of a mixture of ketamine (80 mg/kg) and xylazine (8 mg/kg). For ONT, 0.5% alcaine (Alcon-Couvreur, Puurs, Belgium) was applied to the eyes prior to the surgery, and antiseptic eye drops (Tobres [Tobramycin 0.3%], lcon-Couvreur) were used to prevent infection after the procedures. Finadyne (0.025 mg/mL, Sigma) in drinking water was applied for 7 days to relieve the pain after the surgeries when needed. All animals were sacrificed with overdose of pentobarbital at different time points of interest.

Optic nerve transection (ONT)

For ONT, after the animal was anesthetized by ketamine (80 mg/kg) and xylazine (8 mg/kg), the posterior pole of the eye was exposed through a superior temporal intra-orbital approach. The eyelid was lifted up using a suture, and bulbar conjunctiva was cut coronally to expose the superior extraocular muscles. By lifting up the muscles using forceps, the intraorbital portion of the optic nerve (ON) was exposed and its dura sheath was opened longitudinally. A complete transection was made to the ON at 1.5 mm posterior to the optic disc as previously described. Care was taken to maintain the blood supply to the retina.

The animals were sacrificed 1, 3, 7, 14, 21 days, 6 weeks and 8 weeks after ONT, and retinas were harvested for whole-mount immunohistochemistry.

Retinal whole-mount immunohistochemistry

The retinas were fixed in 4% PFA (Sigma) at room temperature for 1 hour, followed by PBS (Sigma) wash. Then, the retinas were blocked by 0.5% triton X-100, 1% bovine serum albumin (BSA, Sigma) and 10% normal goat serum (Jackson) in PBS at room temperature for 2 hours. After that, they were incubated in a diluted primary antibody solution overnight at room temperature. After sequential PBS wash, they were incubated in secondary antibody solution at room temperature for 2 hours. Finally, the retinas were washed in PBS and mounted with fluorescein mounting medium (Dako). The antibodies used included rabbit anti-Iba1 (1:500, Wako), goat anti-rabbit IgG Alexa Fluor 488 (1:200, Life Technology).


The whole-mount retinas were visualized with a Zeiss Axiophot epi-fluorescence microscope; confocal images (2048 × 2048) were captured by Carl Zeiss LSM 700/710 laser scanning microscopes for analysis. Microglial cells were rendered by green pseudo-color. Both bright field and fluorescent images were collected sequentially.

Preprocessing of confocal images

All image analysis programs were developed in-house on the platform of Matlab 2015a. The image processing toolbox was used to accelerate the program development.

All confocal images of microglial cells were rendered by fluorescent immunohistochemistry staining. The original image sizes are of 2048 × 2048. Three color channels represent Red, Green, and Blue. The data are stored in uint16 format in the range of [0, 65535].

We first transform the data format from uint16 to double, so as to meet the precision requirement of digital image processing. This is accomplished using the “im2double” command.

The color image was then transformed to a grayscale image because the grayscale version contains enough information. Cells take larger grayscale values, and backgrounds take smaller values. This process is accomplished by the “rgb2gray” command.

Next, the log enhancement was applied to improve visual clarity. The log enhancement was achieved by the “log” command.

After log enhancement, the grayscale values of the image may be compressed into a small-scale range. Therefore, we needed to normalize the intensity values to the range of [0, 1]. This is carried out by the “mat2gray” command. After this step, the intensities of the images are normalized to the range from 0.0 (black) to 1.0 (white).


Segmentation partitions the confocal image into two parts (cell and background). This can simplify the image representation. Suppose the original image is I and the threshold is T, then the segmented image S is a binary image with pixel values either 0 (background) or 1 (Microglia cell).

Otsu’s method9 is employed to obtain the optimal threshold. It assumes the image contains two class of pixels with bi-modal histogram, then it can obtain the optimal threshold that separates the two classes, in the criterion that the intra-class variance is minimal and inter-class variance is maximal10. Again, we used the “graythresh” command to accomplish this process.

Mathematical morphology based cell detection

Mathematical morphology (MM) is a computer-science related technique to process geometric structures based on set theory, topology, etc. By MM the cell detection problem can be transformed to a connected component (CC) detection problem.

CCs are detected using the command “bwconncomp”. This function finds all the CCs in the segmented image S. We manually removed CCs with very small areas that reflect noise. Each CC corresponds to a microglia cell. Note that sometimes several cells overlap with each other. In this case, we resort to manual segmentation. If the overlap is severe, we omit these cells from the following analysis.

Pixel connectivity is the way each pixel relates to its neighbors. In the 4-connected neighbor approach, the neighboring pixels are considered to be those which share an edge with the given pixel. In this system, pixel (x, y) has neighbors (x ± 1, y) and (x, y ± 1). In this work, we employed the 8-connected neighbor approach, where a pixel is considered a neighbor if it touches either the edges or the corners of a given pixel. In this system, pixel (x, y) has neighbors (x ± 1, y), (x, y ± 1), and (x ± 1, y ± 1).

Ellipse approximation of microglia

After obtaining all the CCs of the microglia image, we generated an optimal ellipse to approximate each CC. The general parametric form of an ellipse in trigonometry is

where a and b represents the semi-major and semi-minor axis, respectively. (Xc, Yc) represents the center of the ellipse, φ is the angle between the horizontal direction and the major axis of the ellipse. In all, an ellipse has five parameters (a, b, Xc, Yc, φ). We vary the values of these parameters so that the ellipse E approximates the microglia region CC.

where ∩ is the intersection operator, Δ represents the symmetric difference, and || is the cardinality operator, meaning the number of elements in the set. From the above equation, the criterion is that we expect to maximize the overlapping areas of E and CC, while reducing the extra area of E apart from CC.

Area Calculation

The area of CC is calculated as the area of microglia cell. A simple method is to summarize the number of pixels. Considering different patterns of pixels should be weighted differently, we employed an advanced technique that counts the areas of 2 × 2 neighborhood following six rules:

Neighborhood with zero “1” pixel → Area = 0

Neighborhood with one “1” pixel → Area = 1/4

Neighborhood with two adjacent “1” pixels → Area = 1/2

Neighborhood with two diagonal “1” pixels → Area = 3/4

Neighborhood with three “1” pixels → Area = 7/8

Neighborhood will all “1” pixels → Area = 1

Angle analysis of microglia

Remember the parameter φ, defined as the orientation of the microglial cell. Here it has another physical meaning in that it represents the orientation of the microglial cell within the range of [0, 180] degrees. We obtain the φ values of all CCs, and then implemented a count analysis by the “histogram” command.

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